Difference between revisions of "Part:BBa K5117000"

 
 
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<partinfo>BBa_K5117000 short</partinfo>
 
<partinfo>BBa_K5117000 short</partinfo>
  
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ribosome binding site for Bacillus subtilis (already described and tested by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer
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This part consists of the ribosome binding site of <i>Bacillus subtilis</i> (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer.
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BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).
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<b>Biosafety level:</b> S1
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<b>Target organism:</b> <i>Bacillus subtilis</i>
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<b>Main purpose of use:</b> Gene expression and protein production using the host <i>B. subtilis</i>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5117000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5117000 SequenceAndFeatures</partinfo>
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===References===
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Vellanoweth R. L. & Rabinowitz J. C. (1992): The influence of ribosome‐binding‐site elements on translational efficiency in <i>Bacillus subtilis</i> and <i>Escherichia coli in vivo</i>. Molecular microbiology 6(9), 1105-1114.  https://doi.org/10.1111/j.1365-2958.1992.tb01548.x
  
  

Latest revision as of 23:35, 1 October 2024


BsRBS

This part consists of the ribosome binding site of Bacillus subtilis (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer.

BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).


Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Gene expression and protein production using the host B. subtilis


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Vellanoweth R. L. & Rabinowitz J. C. (1992): The influence of ribosome‐binding‐site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Molecular microbiology 6(9), 1105-1114. https://doi.org/10.1111/j.1365-2958.1992.tb01548.x