Difference between revisions of "Part:BBa K5103003:Design"
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===Source===
 
===Source===
  
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The sequence for Cry8Da is sourced from NCBI<sup>[1]</sup>.
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The sequence for Cry8Da is sourced from NCBI.<sup>[1]</sup>
  
 
===References===
 
===References===
−
[1] Xie, B., & Liu, Y. (2013). Cry8Da [Bacillus thuringiensis]. https://www.ncbi.nlm.nih.gov/protein/543174970
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[1] Xie, B., & Liu, Y. (2013). Cry8Da [Bacillus thuringiensis]. <i>NCBI</i>. https://www.ncbi.nlm.nih.gov/protein/543174970

Latest revision as of 23:22, 1 October 2024


Cry8Da with HindIII and XhoI cut sites introduced using primers


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 987
    Illegal EcoRI site found at 1066
    Illegal EcoRI site found at 2478
    Illegal EcoRI site found at 3141
    Illegal PstI site found at 118
    Illegal PstI site found at 1559
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 987
    Illegal EcoRI site found at 1066
    Illegal EcoRI site found at 2478
    Illegal EcoRI site found at 3141
    Illegal PstI site found at 118
    Illegal PstI site found at 1559
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 987
    Illegal EcoRI site found at 1066
    Illegal EcoRI site found at 2478
    Illegal EcoRI site found at 3141
    Illegal XhoI site found at 3446
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 987
    Illegal EcoRI site found at 1066
    Illegal EcoRI site found at 2478
    Illegal EcoRI site found at 3141
    Illegal PstI site found at 118
    Illegal PstI site found at 1559
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 987
    Illegal EcoRI site found at 1066
    Illegal EcoRI site found at 2478
    Illegal EcoRI site found at 3141
    Illegal PstI site found at 118
    Illegal PstI site found at 1559
    Illegal AgeI site found at 1333
    Illegal AgeI site found at 2215
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

HindIII and XhoI cut sites were selected with consideration to multiple cloning site within PYES2.


Source

The sequence for Cry8Da is sourced from NCBI.[1]

References

[1] Xie, B., & Liu, Y. (2013). Cry8Da [Bacillus thuringiensis]. NCBI. https://www.ncbi.nlm.nih.gov/protein/543174970