Difference between revisions of "Part:BBa K5439003"
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===Usage and Biology===
 
===Usage and Biology===
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<b>Table 1</b> displays the protocol followed for a 50 µL reaction.
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__TOC__
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=Usage and Biology=
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<div style="text-align:justify;">
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{| class="wikitable" style="margin:auto; text-align:center; length: 80%"
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|+ Table 1. Restriction digest conditions.
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|-
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!Reactive !! Quantity
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|-
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| style="text-align:center;" style="width: 80%;" | Nuclease-free water || To 50 µL
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|-
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| style="text-align:center;" style="width: 80%;" | rCutSmart Buffer || 5 µL
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|--
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| style="text-align:center;" style="width: 80%;" | Template DNA (up to 4000 ng) || X µL
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|-
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| style="text-align:center;" style="width: 80%;" | <i>NcoI</i> restriction enzyme|| 1 µL
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|-
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| style="text-align:center;" style="width: 80%;" | <i>XhoI</i> restriction enzyme || 1 µL
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|}
  
 
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Revision as of 23:00, 1 October 2024


FRET-based system for the detection of rifampicin

FRET-based sensor system for the detection of rifampicin that consists of rifampicin monooxygenase (K4447003), an enzyme that catalyzes the hydroxylation of rifampicin, flanked by two fluorescent proteins: ECFP (BBa_K1159302) as energy donor and mVenus (BBa_K1907000) as an energy acceptor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1947
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2833