Difference between revisions of "Part:BBa K5246050"

 
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<partinfo>BBa_K5246050 short</partinfo>
 
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TBA
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T===Introduction===
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Vilnius-Lithuania iGEM 2024 project <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">Synhesion</a></b></html> aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - <I> C. crescentus </I> and <I> H. baltica </I> - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used <I>E. coli</I> bacteria to produce adhesives. Our team concomitantly focused on creating a novel <I>E. coli</I> strain for more efficient production of adhesives.
  
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This is a <b>part of the complete holdfast tetrad assembly system </b> <HTML><b><a href="https://parts.igem.org/Part:BBa_K5246046" target="_blank">BBa_K5246043</a></b></html> used in Vilnius-Lithuania iGEM 2024 project "Synhesion" <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">https://2024.igem.wiki/vilnius-lithuania/</a></b></html>
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===Usage and Biology===
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===Functional Parameters===
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<partinfo>BBa_K5246050 parameters</partinfo>
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Revision as of 21:58, 1 October 2024


H.Baltica hfsA-hfsB-hfsD Part of polysaccharide export apparatus

T===Introduction=== Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

This is a part of the complete holdfast tetrad assembly system BBa_K5246043 used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2647
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2186
  • 1000
    COMPATIBLE WITH RFC[1000]