Difference between revisions of "Part:BBa K5246049"
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===Introduction===
 
===Introduction===
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===Introduction===
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Vilnius-Lithuania iGEM 2024 project <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">Synhesion</a></b></html> aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - <I> C. crescentus </I> and <I> H. baltica </I> - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used <I>E. coli</I> bacteria to produce adhesives. Our team concomitantly focused on creating a novel <I>E. coli</I> strain for more efficient production of adhesives.
  
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This is a <b>part of the complete holdfast tetrad assembly system </b> <HTML><b><a href="https://parts.igem.org/Part:BBa_K5246046" target="_blank">BBa_K5246043</a></b></html> used in Vilnius-Lithuania iGEM 2024 project "Synhesion" <HTML><b><a href="https://2024.igem.wiki/vilnius-lithuania" target="_blank">https://2024.igem.wiki/vilnius-lithuania/</a></b></html>
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===Usage and Biology===
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TBA
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K5246049 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5246049 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K5246049 parameters</partinfo>
 
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===Experimental characterization===
 
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===References===
 

Revision as of 21:54, 1 October 2024


H.Baltica hfsE-hfsG-hfsH-hfsJ-hfsL-hfsK Polysaccharide tetrad assembly system


Introduction

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

This is a part of the complete holdfast tetrad assembly system BBa_K5246043 used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3763
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2514
    Illegal PstI site found at 3763
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2409
    Illegal BglII site found at 4197
    Illegal BglII site found at 6366
    Illegal BamHI site found at 135
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3763
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3763
    Illegal NgoMIV site found at 301
    Illegal NgoMIV site found at 3061
    Illegal NgoMIV site found at 4518
    Illegal AgeI site found at 1468
    Illegal AgeI site found at 6416
  • 1000
    COMPATIBLE WITH RFC[1000]