Difference between revisions of "Part:BBa K5492721:Design"

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===Design Notes===
 
===Design Notes===
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complementary part to BBa_K5492731 (H1_JOHNSON_REVCOMP)
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[https://parts.igem.org/Part:BBa_K5492711 J1 ssDNA aptamer] sequence designed to specifically bind to histamine.
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fluorophor /5cy5/ is attached to the start of the sequence
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The sequence contains a fluorophore molecule at its 5' end.
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[https://parts.igem.org/Part:BBa_K5492731 j1_reverse_compliment_strand] is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by fluorophore until the aptamer binds histamine.
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===Source===
 
===Source===
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===References===
 
===References===
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http://www.unafold.org/mfold/applications/dna-folding-form.php - Folding
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Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.
 
Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.

Latest revision as of 21:50, 1 October 2024


J1_aptamer_with_fluorophore


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

J1 ssDNA aptamer sequence designed to specifically bind to histamine. The sequence contains a fluorophore molecule at its 5' end. j1_reverse_compliment_strand is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by fluorophore until the aptamer binds histamine.

Source

https://www.sciencedirect.com/science/article/abs/pii/S0039914019311075?via%3Dihub Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.

References

http://www.unafold.org/mfold/applications/dna-folding-form.php - Folding

Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.