Difference between revisions of "Part:BBa K5492722:Design"
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===References=== | ===References=== | ||
http://www.unafold.org/mfold/applications/dna-folding-form.php - Folding | http://www.unafold.org/mfold/applications/dna-folding-form.php - Folding | ||
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Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075. | Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075. | ||
Latest revision as of 21:48, 1 October 2024
S1_aptamer_with_fluorophore
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
s1 ssDNA aptamer sequence designed to specifically bind to histamine. The sequence contains a fluorophore molecule at its 5' end. s1_reverse_compliment_strand is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by fluorophore until the aptamer binds histamine.
Source
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b00075 Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.
References
http://www.unafold.org/mfold/applications/dna-folding-form.php - Folding
Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.