Difference between revisions of "Part:BBa K5325001:Design"

 
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===References===
 
===References===
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Nakamura, K., Tomita, T., Abe, N., Kamio, Y. (2001). Purification and Characterization of an Extracellular Poly( <span style="font-variant:small-caps;">l</span> -Lactic Acid) Depolymerase from a Soil Isolate, <i>Amycolatopsis</i> sp. Strain K104-1. Appl Environ Microbiol. 67(1):345-353

Revision as of 21:43, 1 October 2024


Amy_plaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 637
    Illegal PstI site found at 71
    Illegal PstI site found at 519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 637
    Illegal PstI site found at 71
    Illegal PstI site found at 519
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 637
    Illegal PstI site found at 71
    Illegal PstI site found at 519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 637
    Illegal PstI site found at 71
    Illegal PstI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C-terminal FLAG tag that helps with protein purification and Western Blot The DNA sequence was codon optimized for expression in E. coli and was found to work in Shewanella oneidensis


Source

This part originated from the genome of Amycolatopsis sp. strain K104-1

References

Nakamura, K., Tomita, T., Abe, N., Kamio, Y. (2001). Purification and Characterization of an Extracellular Poly( l -Lactic Acid) Depolymerase from a Soil Isolate, Amycolatopsis sp. Strain K104-1. Appl Environ Microbiol. 67(1):345-353