Difference between revisions of "Part:BBa K197026:Design"
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===Source=== | ===Source=== | ||
+ | <pre> | ||
Construction of Ice Nucleation Repeats (INP repeats) Basic Part | Construction of Ice Nucleation Repeats (INP repeats) Basic Part | ||
PCR Oeh001F/Oeh002R on pBca1256-Bca1346 (2296bp, EcoRI/BamHI) | PCR Oeh001F/Oeh002R on pBca1256-Bca1346 (2296bp, EcoRI/BamHI) | ||
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eh001R Reverse BamHI oligo for <INP repeats> | eh001R Reverse BamHI oligo for <INP repeats> | ||
gtcagGGATCCactgtcctgcccggccgttg | gtcagGGATCCactgtcctgcccggccgttg | ||
+ | </pre> | ||
===References=== | ===References=== | ||
Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340 | Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340 |
Revision as of 04:15, 22 October 2009
INP-Repeats
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 43
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a part of a repeat in the ice nucleation protien
Source
Construction of Ice Nucleation Repeats (INP repeats) Basic Part PCR Oeh001F/Oeh002R on pBca1256-Bca1346 (2296bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, L) Product is pBca9495AK-M10048 {<INP repeats>} --- eh001F Forward EcoRI oligo for <INP repeats> gtcagGAATTCatgAGATCTaaccacagtgatctaattgc eh001R Reverse BamHI oligo for <INP repeats> gtcagGGATCCactgtcctgcccggccgttg
References
Kawahara H. The structures and functions of ice crystal-controlling proteins from bacteria. J Biosci Bioeng. 2002;94(6):492-6. PMID: 16233340