Difference between revisions of "Part:BBa K247004:Experience"
(→User Reviews) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 30: | Line 30: | ||
*AHL concentration of medium : 100 nM | *AHL concentration of medium : 100 nM | ||
| − | *Time when we observed mRFP fluorescence : 24 h | + | *Time when we observed mRFP fluorescence : 24 h |
| − | *medium : 1(left) or 5 | + | *medium : 10 mL of LB-Amp,Cm medium containing 1(left) or 5(right) %(w/v) agar |
[[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1 RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]] | [[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1 RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]] | ||
Latest revision as of 04:09, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K247004
User Reviews
UNIQec472a11a6e52991-partinfo-00000000-QINU UNIQec472a11a6e52991-partinfo-00000001-QINU
This part properly acted as RFP reporter.
Excitation peak: 584 nm, Emission peak: 607 nm
The Chiba iGEM team tested this part in several conditions.
Experiment Condition
- AHL concentration of medium : 100 nM
- Time when we observed mRFP fluorescence : 24 h
- medium : 10 mL of LB-Amp,Cm medium containing 1(left) or 5(right) %(w/v) agar
