Difference between revisions of "Part:BBa K193001"

Latest revision as of 04:08, 22 October 2009

CI repressible LuxI enzyme generator

CI repressible LuxI enzyme generator.

This part is used to transform E.coli strain which expresses CI represser.

We improved existing BioBrick Part BBa_F1610, and constructed K193001.

luxR(AHL+),luxR(AHL-) ; strain N4830 transformed only with luxR expressing plasmid(BBa_J54140)

luxR+luxI; strain N4830 transformed with luxR expressing plasmid and luxI expressing plasmid

luxI; strain N4830 transformed with luxI expressing plasmid

More detail information, http://2009.igem.org/Team:Tokyo_Tech/Consortium.

Sequence and Features

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K193001 short</partinfo>
-cI repressible LuxI enzyme generator.
+CI repressible LuxI enzyme generator.
-This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively.
+This part is used to transform E.coli strain which expresses CI represser.
-This part is used to transform E.coli strain N99 and N4830, which express cI and temperature-sensitive cI respectively.
+We improved existing BioBrick Part [https://parts.igem.org/Part:BBa_F1610 BBa_F1610], and constructed K193001.
-We improved existing BioBrick Part F1610(https://parts.igem.org/Part:BBa_F1610), and constructed K193001.
+[[Image:tokyo_tech_consortium11.png|the vertical line is fluorescence intensity (a.u.)]]
+luxR(AHL+),luxR(AHL-) ; strain N4830 transformed only with luxR expressing plasmid(BBa_J54140)
+luxR+luxI; strain N4830 transformed with luxR expressing plasmid and luxI expressing plasmid
+luxI; strain N4830 transformed with luxI expressing plasmid
+More detail information, http://2009.igem.org/Team:Tokyo_Tech/Consortium.