Difference between revisions of "Part:BBa K247004:Experience"

(User Reviews)
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*AHL concentration of medium : 100 nM
 
*AHL concentration of medium : 100 nM
  
*Time when we observed mRFP fluorescence : 24 h (since we permeated AHL to this RFP)
+
*Time when we observed mRFP fluorescence : 24 h
  
*medium : 1(left) or 5%(right) agar and 10 mL of  LB-Amp, Cm medium
+
*medium : 10 mL ofLB-Amp,Cm medium containing 1(left) or 5%(right) agar
  
 
[[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1 RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]]
 
[[Image:Chiba_BBa_K247004.png|thumb|left|'''Fig. 1 RFP Fluorescent'''. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.]]

Revision as of 04:08, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K247004

User Reviews

UNIQ5a726fff5309da24-partinfo-00000000-QINU UNIQ5a726fff5309da24-partinfo-00000001-QINU

This part properly acted as RFP reporter.

Excitation peak: 584 nm, Emission peak: 607 nm


The Chiba iGEM team tested this part in several conditions.

Experiment Condition

  • AHL concentration of medium : 100 nM
  • Time when we observed mRFP fluorescence : 24 h
  • medium : 10 mL ofLB-Amp,Cm medium containing 1(left) or 5%(right) agar
Fig. 1 RFP Fluorescent. RFP expression is induced by AHL (100 nM). Observed under UV irradiation.