Difference between revisions of "Collections/VEGFR-like"
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Revision as of 19:36, 1 October 2024
VEGFR-like
This year, the USTC iGEM teamlianjiewenben has utilized the competitive binding of vascular endothelial growth factor (VEGF) to develop a targeted bacterial therapy for solid tumors. Our quest for the optimal VEGF-binding protein(or peptide) led us to an in-depth exploration of proteins structurally akin to the vascular endothelial growth factor receptor (VEGFR), which we have named VEGFR-like. In this year's project, we selected pBBR1MCS-2 as our plasmid backbone to construct a series of plasmids, which are used to validate the surface display system, VEGFR-like, and the masking peptides targeting VEGFR-like. And we have verified the expression of several of these plasmids in EcN.
| Name | Type | Description | Designer | Length |
| BBa_K5302000 | Coding | VEGFR1D2 | Jiacan Ma | 303 |
| BBa_K5302001 | Coding | 8IIU | Jiacan Ma | 378 |
| BBa_K5302002 | Coding | 8IJZ | Jiacan Ma | 378 |
| BBa_K5302003 | Coding | ZVEGF | Dekun Zhou | 177 |
| BBa_K5302004 | Coding | Z3C | Dekun Zhou | 177 |
| BBa_K5302005 | Coding | miniZ | Dekun Zhou | 102 |
| BBa_K5302006 | Coding | V114 | Dekun Zhou | 57 |
| BBa_K5302007 | Coding | V107 | Dekun Zhou | 57 |
| BBa_K5302008 | Coding | VEGFR-masking#18 | Dekun Zhou | 93 |
| BBa_K5302009 | Coding | VEGFR-masking#29 | Dekun Zhou | 84 |
| BBa_K5302010 | Coding | VGB | Dekun Zhou | 96 |
| BBa_K5302011 | Plasmid | pBBR-OmpA-mCherry | Jiacan Ma | 6483 |
| BBa_K5302012 | Plasmid | pBBR-INP-mcherry | Jiacan Ma | 6799 |
| BBa_K5302013 | Plasmid | pBBR-LppOmpA-mCherry | Bojun Yang | 5940 |
| BBa_K5302014 | Plasmid | pBBR-intimin-mCherry | Bojun Yang | 6957 |
| BBa_K5302015 | Plasmid | pBBR-AIDA-mCherry | Bojun Yang | 7269 |
| BBa_K5302016 | Plasmid | pBBR-OmpA-VEGFR1D2 | Xinyuan Shi | 6150 |
| BBa_K5302017 | Plasmid | pBBR-OmpA-8IIU | Xinyuan Shi | 6225 |
| BBa_K5302018 | Plasmid | pBBR-OmpA-8IJZ | Xinyuan Shi | 6225 |
| BBa_K5302019 | Plasmid | pBBR-OmpA-ZVEGF | Xinyuan Shi | 6024 |
| BBa_K5302020 | Plasmid | pBBR-OmpA-Z3C | Xinyuan Shi | 6024 |
| BBa_K5302021 | Plasmid | pBBR-OmpA-miniZ | Xinyuan Shi | 5949 |
| BBa_K5302022 | Plasmid | pBBR-OmpA-V114 | Xinyuan Shi | 5903 |
| BBa_K5302023 | Plasmid | pBBR-OmpA-V107 | Xinyuan Shi | 5903 |
| BBa_K5302024 | Plasmid | pBBR-INP-VEGFR1D2 | Bojun Yang | 6466 |
| BBa_K5302025 | Plasmid | pBBR-INP-8IIU | Bojun Yang | 6541 |
| BBa_K5302026 | Plasmid | pBBR-INP-8IJZ | Bojun Yang | 6541 |
| BBa_K5302027 | Plasmid | pBBR-INP-ZVEGF | Bojun Yang | 6340 |
| BBa_K5302028 | Plasmid | pBBR-INP-Z3C | Bojun Yang | 6340 |
| BBa_K5302029 | Plasmid | pBBR-INP-miniZ | Bojun Yang | 6265 |
| BBa_K5302030 | Plasmid | pBBR-INP-V114 | Bojun Yang | 6220 |
| BBa_K5302031 | Plasmid | pBBR-INP-V107 | Bojun Yang | 6220 |
| BBa_K5302032 | Plasmid | pBBR-OmpA-VEGFR1D2-l1masking#18 | Dekun Zhou | 6144 |
| BBa_K5302033 | Plasmid | pBBR-OmpA-VEGFR1D2-l2masking#18 | Dekun Zhou | 6144 |
| BBa_K5302034 | Plasmid | pBBR-OmpA-VEGFR1D2-l1masking#29 | Dekun Zhou | 6135 |
| BBa_K5302035 | Plasmid | pBBR-OmpA-VEGFR1D2-l2masking#29 | Dekun Zhou | 6135 |
| BBa_K5302036 | Plasmid | pBBR-OmpA-l1VGB | Dekun Zhou | 6147 |
| BBa_K5302037 | Plasmid | pBBR-OmpA-l2VGB | Dekun Zhou | 6147 |
| BBa_K5302038 | Plasmid | pBBR-INP-VEGFR1D2-l1masking#18 | Dekun Zhou | 6496 |
| BBa_K5302039 | Plasmid | pBBR-INP-VEGFR1D2-l2masking#18 | Dekun Zhou | 6496 |
| BBa_K5302040 | Plasmid | pBBR-INP-VEGFR1D2-l1masking#29 | Dekun Zhou | 6487 |
| BBa_K5302041 | Plasmid | pBBR-INP-VEGFR1D2-l2masking#29 | Dekun Zhou | 6487 |
| BBa_K5302042 | Plasmid | pBBR-INP-l1VGB | Dekun Zhou | 6499 |
| BBa_K5302043 | Plasmid | pBBR-INP-l2VGB | Dekun Zhou | 6499 |
Reference
[1]Ivan Guryanov*, Viktor Korzhikov-Vlakh, Madhushree Bhattacharya, Barbara Biondi, Giulia Masiero, Fernando Formaggio, Tatiana Tennikova, Arto Urtt. Conformationally Constrained Peptides with High Affinity to the Vascular Endothelial Growth Factor.Journal of Medicinal Chemistry. Vol 64/Issue 15.July 16, 2021
[2]Pan B, Li B, Russell SJ, Tom JY, Cochran AG, Fairbrother WJ. Solution structure of a phage-derived peptide antagonist in complex with vascular endothelial growth factor. J Mol Biol. 2002 Feb 22;316(3):769-87. doi: 10.1006/jmbi.2001.5370
[3]Checco JW, Kreitler DF, Thomas NC, Belair DG, Rettko NJ, Murphy WL, Forest KT, Gellman SH. Targeting diverse protein-protein interaction interfaces with α/β-peptides derived from the Z-domain scaffold. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4552-7. doi: 10.1073/pnas.1420380112
[4]Rossella Di Stasi, Donatella Diana, Domenica Capasso, Rosanna Palumbo, Alessandra Romanelli, Carlo Pedone, Roberto Fattorusso, Luca D. D'Andrea. VEGFR1D2 in drug discovery: Expression and molecular characterization.19 November 2010
[5]Anna Fedorova, Kerry Zobel, Herman S. Gill, Annie Ogasawara, Judith E. Flores, Jeff N. Tinianow, Alexander N. Vanderbilt, Ping Wu, Y. Gloria Meng, Simon-P. Williams, Christian Wiesmann, Jeremy Murray, Jan Marik, Kurt Deshayes. The Development of Peptide-Based Tools for the Analysis of Angiogenesis. Chemistry & Biology. Volume 18, Issue 7. 2011. Pages 839-845. ISSN 1074-5521
[6]Balsera B, Bonache MÁ, Reille-Seroussi M, Gagey-Eilstein N, Vidal M, González-Muñiz R, Pérez de Vega MJ. Disrupting VEGF-VEGFR1 Interaction: De Novo Designed Linear Helical Peptides to Mimic the VEGF13-25 Fragment. Molecules. 2017 Oct 28;22(11):1846
[7]Lei Wang,Lingyu Zhou,Marie Reille-Seroussi,Nathalie Gagey-Eilstein,Sylvain Broussy, Tianyu Zhang,Lili Ji,Michel Vidal*,Wang-Qing Liu.Identification of Peptidic Antagonists of Vascular Endothelial Growth Factor Receptor 1 by Scanning the Binding Epitopes of Its Ligands.July7.2017
[8]Sonia Nicchi1,2, Maria Giuliani1 , Fabiola Giusti1 , Laura Pancotto1 , Domenico Maione1 , Isabel Delany1 ,
Cesira L. Galeotti1 and Cecilia Brettoni1*.Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of diferent display systems.2021