Difference between revisions of "Part:BBa K193001"
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We improved existing BioBrick Part [https://parts.igem.org/Part:BBa_F1610 BBa_F1610], and constructed K193001. | We improved existing BioBrick Part [https://parts.igem.org/Part:BBa_F1610 BBa_F1610], and constructed K193001. | ||
| − | [[Image:tokyo_tech_consortium11.png]] | + | [[Image:tokyo_tech_consortium11.png|the vertical line is fluorescence intensity (a.u.)]] |
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| + | luxR(AHL+),luxR(AHL-) ; strain N4830 transformed only with luxR expressing plasmid(BBa_J54140) | ||
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| + | luxR+luxI; strain N4830 transformed with luxR expressing plasmid and luxI expressing plasmid | ||
| + | |||
| + | luxI; strain N4830 transformed with luxI expressing plasmid | ||
| + | |||
Revision as of 04:04, 22 October 2009
CI repressible LuxI enzyme generator
CI repressible LuxI enzyme generator.
This part is used to transform E.coli strain which expresses CI represser.
We improved existing BioBrick Part BBa_F1610, and constructed K193001.
luxR(AHL+),luxR(AHL-) ; strain N4830 transformed only with luxR expressing plasmid(BBa_J54140)
luxR+luxI; strain N4830 transformed with luxR expressing plasmid and luxI expressing plasmid
luxI; strain N4830 transformed with luxI expressing plasmid
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 712
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]