Difference between revisions of "Part:BBa K265009"

Latest revision as of 04:01, 22 October 2009

Ice Nucleation Protein NC + Proteolytic Cleavage Sequence

Park et al. have shown that the fusion of the complete phaZ1 gene (including cleavage sequence) and a truncated ice nucleation protein from Pseudomonas syringae (BBa_K265008), could lead to stable expression and secretion of the phaZ1 gene product.

We propose that this system might be generally useful for the secretion of other target proteins in E. coli and have therefore created a fusion of parts BBa_K265008 (INPNC) and BBa_K265002 (cleavage sequence) which is compatible with the BBF RFC-12 Standard.

During the construction of this part, two silent mutations were introduced in the coding region of INPNC (T324A and A348T) that differ from those in part BBa_K265008.

This part is authored by Richard Hauser, Tim Fenton, Willis Wong, Lydia Kwon, Jimmy Huang, and Pantea Gheibi. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 405
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K265009 short</partinfo>
-It is believed that when coupled with the correct signal sequence, INPNC will secrete better.
+Park et al. have shown that the fusion of the complete phaZ1 gene (including cleavage sequence) and a truncated ice nucleation protein from Pseudomonas syringae (BBa_K265008), could lead to stable expression and secretion of the phaZ1 gene product.
+We propose that this system might be generally useful for the secretion of other target proteins in E. coli and have therefore created a fusion of parts BBa_K265008 (INPNC) and BBa_K265002 (cleavage sequence) which is compatible with the BBF RFC-12 Standard.
+During the construction of this part, two silent mutations were introduced in the coding region of INPNC (T324A and A348T) that differ from those in part BBa_K265008.
+This part is authored by Richard Hauser, Tim Fenton, Willis Wong, Lydia Kwon, Jimmy Huang, and Pantea Gheibi.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===