Difference between revisions of "Part:BBa I731012:Experience"

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The Chiba iGEM team tested this part in several conditions.
 
*Without LuxI (or synthetic OHHL), RFP levels increase.
 
*Without LuxI (or synthetic OHHL), RFP levels increase.
  
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#Picked and cultured the following stab and glycerol stocks in 2mL of LB:
 
#Picked and cultured the following stab and glycerol stocks in 2mL of LB:
 
##LB-Amp, BBa_I731012, (stab, received from iGEM)
 
##LB-Amp, BBa_I731012, (stab, received from iGEM)
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##LB-Amp+0.2%Glu, BBa_K084007(plac+rbs+LasI(no LVA)), (JW1998)
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##LB-Amp, BBa_K084007(plac+rbs+LasI(no LVA)), (JW1908)
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##LB-Amp+0.2%Glu, BBa_K084012(plac+rbs+LuxI(no LVA)), (JW1998)
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##LB-Amp, BBa_K084012(plac+rbs+LuxI(no LVA)), (JW1908)
 
#Pre-Cultured at 37°C for 12h.
 
#Pre-Cultured at 37°C for 12h.
 
#Added 6.25% each of the pre-cultures to new LB medium.
 
#Added 6.25% each of the pre-cultures to new LB medium.

Latest revision as of 03:55, 22 October 2009

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Applications of BBa_I731012

User Reviews

UNIQfbe362131ce47288-partinfo-00000000-QINU UNIQfbe362131ce47288-partinfo-00000001-QINU

The Chiba iGEM team tested this part in several conditions.

  • Without LuxI (or synthetic OHHL), RFP levels increase.

Experiment : checking BBa_I731012

  1. Picked and cultured the following stab and glycerol stocks in 2mL of LB:
    1. LB-Amp, BBa_I731012, (stab, received from iGEM)
    2. LB-Amp, BBa_K084007(plac+rbs+LasI(no LVA)), (JW1908)
    3. LB-Amp, BBa_K084012(plac+rbs+LuxI(no LVA)), (JW1908)
  2. Pre-Cultured at 37°C for 12h.
  3. Added 6.25% each of the pre-cultures to new LB medium.
    1. LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
  4. Cultured at 37°C for 12h
  5. Mixed the sender cells BBa_K08400 and 7BBa_K084012 both with BBa_I1731012 at a 1:1 ratio.
    1. BBa_I1731012 alone.
    2. BBa_K084007:BBa_BBa_I1731012=1:1
    3. BBa_K084012:BBa_I1731012=1:1
  6. Culture 12h and observe results(follow picture).

  • Funakoshi, UVGL-58 : long wave(365 nm)