Difference between revisions of "Part:BBa I731012:Experience"

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The Chiba iGEM team tested this part in several conditions.
 +
*Without LuxI (or synthetic OHHL), RFP levels increase.
 +
 
Experiment : checking  BBa_I731012
 
Experiment : checking  BBa_I731012
 
#Picked and cultured the following stab and glycerol stocks in 2mL of LB:
 
#Picked and cultured the following stab and glycerol stocks in 2mL of LB:
 
##LB-Amp, BBa_I731012, (stab, received from iGEM)
 
##LB-Amp, BBa_I731012, (stab, received from iGEM)
−
##LB-Amp+0.2%Glu, BBa_K084012(plac+rbs+LuxI(no LVA)), (XL10G)
+
##LB-Amp, BBa_K084007(plac+rbs+LasI(no LVA)), (JW1908)
−
##LB-Amp+0.2%Glu, BBa_K084007(plac+rbs+LasI(no LVA)), (XL10G)
+
##LB-Amp, BBa_K084012(plac+rbs+LuxI(no LVA)), (JW1908)
 
#Pre-Cultured at 37°C for 12h.
 
#Pre-Cultured at 37°C for 12h.
 
#Added 6.25% each of the pre-cultures to new LB medium.
 
#Added 6.25% each of the pre-cultures to new LB medium.
 
##LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
 
##LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
 
#Cultured at 37°C for 12h
 
#Cultured at 37°C for 12h
−
#Mixed the sender cells BBa_K084012 and BBa_K084007 both with BBa_I1731012 at a 1:1 ratio.
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#Mixed the sender cells BBa_K08400 and 7BBa_K084012 both with BBa_I1731012 at a 1:1 ratio.
 
##BBa_I1731012 alone.
 
##BBa_I1731012 alone.
−
##BBa_K084012:BBa_I1731012=1:1
 
 
##BBa_K084007:BBa_BBa_I1731012=1:1
 
##BBa_K084007:BBa_BBa_I1731012=1:1
 +
##BBa_K084012:BBa_I1731012=1:1
 
#Culture 12h and observe results(follow picture).
 
#Culture 12h and observe results(follow picture).
−
*Funakoshi, UVGL-58 : long wave(365 nm)
 
 
<html><a href="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" width="400" height="300"></a></html>
 
<html><a href="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" width="400" height="300"></a></html>
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*Funakoshi, UVGL-58 : long wave(365 nm)

Latest revision as of 03:55, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I731012

User Reviews

UNIQa88609945fa8a51d-partinfo-00000000-QINU UNIQa88609945fa8a51d-partinfo-00000001-QINU

The Chiba iGEM team tested this part in several conditions.

  • Without LuxI (or synthetic OHHL), RFP levels increase.

Experiment : checking BBa_I731012

  1. Picked and cultured the following stab and glycerol stocks in 2mL of LB:
    1. LB-Amp, BBa_I731012, (stab, received from iGEM)
    2. LB-Amp, BBa_K084007(plac+rbs+LasI(no LVA)), (JW1908)
    3. LB-Amp, BBa_K084012(plac+rbs+LuxI(no LVA)), (JW1908)
  2. Pre-Cultured at 37°C for 12h.
  3. Added 6.25% each of the pre-cultures to new LB medium.
    1. LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
  4. Cultured at 37°C for 12h
  5. Mixed the sender cells BBa_K08400 and 7BBa_K084012 both with BBa_I1731012 at a 1:1 ratio.
    1. BBa_I1731012 alone.
    2. BBa_K084007:BBa_BBa_I1731012=1:1
    3. BBa_K084012:BBa_I1731012=1:1
  6. Culture 12h and observe results(follow picture).

  • Funakoshi, UVGL-58 : long wave(365 nm)