Difference between revisions of "Part:BBa K5401004:Design"
 
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===References===
 
===References===
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Liang, X., Li, C., Wang, W., & Li, Q. (2018). Integrating T7 RNA Polymerase and Its Cognate Transcriptional Units for a Host-Independent and Stable Expression System in Single Plasmid. ACS Synthetic Biology, 7(5), 1424–1435. https://doi.org/10.1021/acssynbio.8b00055
 +
 +
Vogel, J., & Luisi, B. F. (2011). Hfq and its constellation of RNA. Nature reviews. Microbiology, 9(8), 578–589. https://doi.org/10.1038/nrmicro2615

Latest revision as of 17:45, 1 October 2024


Hfq scaffold sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 80
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

No design considerations.

Source

The sequence was ordered as a gBlock from IDT, together with other elements (ie. ASO targeted against T7RNAP mRNA; and lac promoter).

References

Liang, X., Li, C., Wang, W., & Li, Q. (2018). Integrating T7 RNA Polymerase and Its Cognate Transcriptional Units for a Host-Independent and Stable Expression System in Single Plasmid. ACS Synthetic Biology, 7(5), 1424–1435. https://doi.org/10.1021/acssynbio.8b00055

Vogel, J., & Luisi, B. F. (2011). Hfq and its constellation of RNA. Nature reviews. Microbiology, 9(8), 578–589. https://doi.org/10.1038/nrmicro2615