Difference between revisions of "Part:BBa K5175030"

 
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<partinfo>BBa_K5175030 short</partinfo>
 
<partinfo>BBa_K5175030 short</partinfo>
  
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It is a composite component consisting of the T7 promoter, T7 terminator, target genes tphA2, tphA3, tphA1, tphB. It is responsible for converting TPA to 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylic acid (DCD) and oxidising the diol moiety (two hydroxyl groups) of DCD to a keto group to result in the production of PCA .
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It is a composite component consisting of the T7 promoter, lac operator, target genes PETase-MHETase. It is responsible for enabling E.coli to degrade PET.
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Revision as of 17:25, 1 October 2024


T7 promoter-lac operator-pelB-FAST-PETase-G4S-MHETase-T7 terminator

It is a composite component consisting of the T7 promoter, lac operator, target genes PETase-MHETase. It is responsible for enabling E.coli to degrade PET.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2820
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1528
    Illegal XhoI site found at 2786
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
    Illegal NgoMIV site found at 166
    Illegal NgoMIV site found at 1327
    Illegal NgoMIV site found at 1715
    Illegal NgoMIV site found at 2078
  • 1000
    COMPATIBLE WITH RFC[1000]