Difference between revisions of "Part:BBa K5335023"
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<partinfo>BBa_K5335023 short</partinfo> | <partinfo>BBa_K5335023 short</partinfo> | ||
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<div class="part_title">Salicylic acid biosensor</div> | <div class="part_title">Salicylic acid biosensor</div> | ||
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<p> | <p> | ||
To sense the salicylic acid signal produced by plants due to disease, we designd salicylic acid sensors. | To sense the salicylic acid signal produced by plants due to disease, we designd salicylic acid sensors. | ||
| − | The LysR-type regulator NahR senses salicylic acid signals in the environment. | + | The LysR-type regulator NahR senses salicylic acid signals in the environment. |
When the salicylic acid concentration reaches a threshold, the Psal promoter is activated to express the HrpR as well as some shRNAs. | When the salicylic acid concentration reaches a threshold, the Psal promoter is activated to express the HrpR as well as some shRNAs. | ||
| − | <img src="https://static.igem.wiki/teams/5335/nahr/design-sa-circuit.png" style="width: | + | <center><img src="https://static.igem.wiki/teams/5335/nahr/design-sa-circuit.png" style="width:50%; "></center> |
<span> <b>Figure 1. Salicylic acid biosensor circuit</b></span> | <span> <b>Figure 1. Salicylic acid biosensor circuit</b></span> | ||
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</p> | </p> | ||
<br> | <br> | ||
Revision as of 17:11, 1 October 2024
A part (including a reporter gene) that induces and regulates downstream gene expression by salicyli
To sense the salicylic acid signal produced by plants due to disease, we designd salicylic acid sensors. The LysR-type regulator NahR senses salicylic acid signals in the environment. When the salicylic acid concentration reaches a threshold, the Psal promoter is activated to express the HrpR as well as some shRNAs.
" style="width:50%; ">Figure 1. Salicylic acid biosensor circuit
HrpR is one of the components of the heterodimer and is involved in the regulation of downstream circuits in a cascade.[hrpRS文献] shRNAs were designed to target key genes in the nematode growth cycle and were packaged in the interior of the VLP.
We plan to use different concentrations of salicylic acid standards to induce engineered bacteria,and verify the NahR and promoter activity with fluorescent proteins as reporter genes.
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Functional Parameters
Introduction
Usage and Biology