Difference between revisions of "Part:BBa K5302001"

 
 
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<partinfo>BBa_K5302001 short</partinfo>
 
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8IIU is an anti-VEGF nanobody, and it has 2 chains, chainA sequence: mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggykyd avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. chainB sequence:mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggyky avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. These two chains all show great affinity with VEGF, we used pBBR plasmid as a backbone and transfered 8IIU into Escherichia coli Nissle 1917, and finally succeeded in expressing 8IIU.
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This year, the USTC iGEM team has utilized the competitive binding of vascular endothelial growth factor (VEGF) to develop a targeted bacterial therapy for solid tumors. Our quest for the optimal VEGF-binding protein(or peptide) led us to an in-depth exploration of proteins structurally akin to the vascular endothelial growth factor receptor (VEGFR), which we have named VEGFR-like.8IIU is an anti-VEGF nanobody, and it has 2 chains, chainA sequence: mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggykyd avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. chainB sequence:mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggyky avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. These two chains all show great affinity with VEGF, we used pBBR plasmid as a backbone and transfered 8IIU into Escherichia coli Nissle 1917, and finally succeeded in expressing 8IIU.
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-8iiu-1.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 1. </b> 8IIU chainA features, from genbank
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        </caption>
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    </div>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-8iiu-2.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 2. </b> 8IIU chainB features, from genbank
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        </caption>
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    </div>
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</div>
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<html>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-8iiu-3.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 3. </b> 8IIU structure, from genbank
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        </caption>
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    </div>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-8iiu-4.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 4. </b> Colony PCR results of pBBR-OmpA-8IIU
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        </caption>
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    </div>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-8iiu-5.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 5. </b> Colony PCR results of pBBR-INP-8IIU (1)
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        </caption>
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    </div>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-8iiu-6.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 6. </b> Colony PCR results of pBBR-INP-8IIU (2)
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        </caption>
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    </div>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-8iiu-7.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 7. </b> Colony PCR results of pBBR-INP-8IIU (3)
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        </caption>
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    </div>
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</div>
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<html>
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-8iiu-8.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 8. </b> SDS-PAGE analysis of pBBR1MCS-OmpA-8IIU expression in Escherichia coli Nissle 1917
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        </caption>
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    </div>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:55, 1 October 2024


8IIU

This year, the USTC iGEM team has utilized the competitive binding of vascular endothelial growth factor (VEGF) to develop a targeted bacterial therapy for solid tumors. Our quest for the optimal VEGF-binding protein(or peptide) led us to an in-depth exploration of proteins structurally akin to the vascular endothelial growth factor receptor (VEGFR), which we have named VEGFR-like.8IIU is an anti-VEGF nanobody, and it has 2 chains, chainA sequence: mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggykyd avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. chainB sequence:mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggyky avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. These two chains all show great affinity with VEGF, we used pBBR plasmid as a backbone and transfered 8IIU into Escherichia coli Nissle 1917, and finally succeeded in expressing 8IIU.

Jamboree Program
Figure 1. 8IIU chainA features, from genbank

Jamboree Program
Figure 2. 8IIU chainB features, from genbank

Jamboree Program
Figure 3. 8IIU structure, from genbank

Jamboree Program
Figure 4. Colony PCR results of pBBR-OmpA-8IIU

Jamboree Program
Figure 5. Colony PCR results of pBBR-INP-8IIU (1)

Jamboree Program
Figure 6. Colony PCR results of pBBR-INP-8IIU (2)

Jamboree Program
Figure 7. Colony PCR results of pBBR-INP-8IIU (3)

Jamboree Program
Figure 8. SDS-PAGE analysis of pBBR1MCS-OmpA-8IIU expression in Escherichia coli Nissle 1917


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]