Difference between revisions of "Part:BBa K227012"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part, when combined with part BBa_I15010 (Cph8) placed under control of the puc promoter (part BBa_K227007), allows for improved light-sensitive regulation of the LHII complexes in R. sphaeroides, leading to greater light efficient growth[http://2009.igem.org/Team:Wash_U]. | This part, when combined with part BBa_I15010 (Cph8) placed under control of the puc promoter (part BBa_K227007), allows for improved light-sensitive regulation of the LHII complexes in R. sphaeroides, leading to greater light efficient growth[http://2009.igem.org/Team:Wash_U]. | ||
| − | + | In this system, Cph8 will be expressed constitutively under low oxygen tension,and under low light conditions will lead to phosphorylation of OmpR, activating transcription of pucBA (LHII) from the ompC promoter. | |
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Latest revision as of 03:39, 22 October 2009
RBS34+OmpR(sphaeroides)+Term+ompC+pucBA
intermediate part, used in conjunction with Cph8 to control LHII production in R. sphaeroides.
Usage and Biology
This part, when combined with part BBa_I15010 (Cph8) placed under control of the puc promoter (part BBa_K227007), allows for improved light-sensitive regulation of the LHII complexes in R. sphaeroides, leading to greater light efficient growth[http://2009.igem.org/Team:Wash_U]. In this system, Cph8 will be expressed constitutively under low oxygen tension,and under low light conditions will lead to phosphorylation of OmpR, activating transcription of pucBA (LHII) from the ompC promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 587
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 629
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]