Difference between revisions of "Part:BBa K227012"

Latest revision as of 03:39, 22 October 2009

RBS34+OmpR(sphaeroides)+Term+ompC+pucBA

intermediate part, used in conjunction with Cph8 to control LHII production in R. sphaeroides.

Usage and Biology

This part, when combined with part BBa_I15010 (Cph8) placed under control of the puc promoter (part BBa_K227007), allows for improved light-sensitive regulation of the LHII complexes in R. sphaeroides, leading to greater light efficient growth[http://2009.igem.org/Team:Wash_U]. In this system, Cph8 will be expressed constitutively under low oxygen tension,and under low light conditions will lead to phosphorylation of OmpR, activating transcription of pucBA (LHII) from the ompC promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 587
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 629
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 intermediate part, used in conjunction with Cph8 to control LHII production in R. sphaeroides.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-This part, when combined with part BBa_I15010 (Cph8), allows for improved light regulation of the LHII complexes in R. sphaeroides.
+This part, when combined with part BBa_I15010 (Cph8) placed under control of the puc promoter (part BBa_K227007), allows for improved light-sensitive regulation of the LHII complexes in R. sphaeroides, leading to greater light efficient growth[http://2009.igem.org/Team:Wash_U].
+In this system, Cph8 will be expressed constitutively under low oxygen tension,and under low light conditions will lead to phosphorylation of OmpR, activating transcription of pucBA (LHII) from the ompC promoter.
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