Difference between revisions of "Part:BBa K247003:Experience"

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The Chiba iGEM team tested this part in several conditions.
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This mutant LuxR have similar speed of GFP expression of wild type LuxR but have different AHL concentration starting communication of wild type.
 
This mutant LuxR have similar speed of GFP expression of wild type LuxR but have different AHL concentration starting communication of wild type.
  
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The part was tested using this [ protocol].
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How the mutant LuxR was made is refer to [http://2009.igem.org/Making_delayed-LuxR_mutants this link].
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[[Image:Chiba_BBa_K247003.png|thumb|left|'''Fig. 1 Time-course color development on the plate with fixed (100 nM) conc. of AHL.''' Left is wild type LuxR, right is mutant LuxR(Dull). Mutant LuxR obviously shows delay.]]
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[[Image:Chiba_BBa_K247003.png]]
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[[Image:Chiba-Mutants-conc9.png‎|thumb|left|'''Fig. 2 Response threshold of AHL concentration.''' Wild type luxR is induced by 10 nM AHL. LuxR mutant containing this part show AHL threshold at about 100 nM.]]

Latest revision as of 03:39, 22 October 2009

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Applications of BBa_K247003

User Reviews

UNIQ5e8bc45a3c69bd60-partinfo-00000000-QINU UNIQ5e8bc45a3c69bd60-partinfo-00000001-QINU The Chiba iGEM team tested this part in several conditions.

This mutant LuxR have similar speed of GFP expression of wild type LuxR but have different AHL concentration starting communication of wild type.

How the mutant LuxR was made is refer to [http://2009.igem.org/Making_delayed-LuxR_mutants this link].

Fig. 1 Time-course color development on the plate with fixed (100 nM) conc. of AHL. Left is wild type LuxR, right is mutant LuxR(Dull). Mutant LuxR obviously shows delay.


Fig. 2 Response threshold of AHL concentration. Wild type luxR is induced by 10 nM AHL. LuxR mutant containing this part show AHL threshold at about 100 nM.