Difference between revisions of "Part:BBa K5117037"
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<ul>
 
<ul>
−
<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021) </i>
+
<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021) </li>
  
−
<li>ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000) </i>
+
<li>ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000) </li>
  
 
<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4),
 
<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4),
  
−
<div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025) </i></div>
+
<div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025) </li></div>
  
 
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022) </li>
 
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022) </li>

Revision as of 15:03, 1 October 2024


PcotYZ-BsRBS-BsEglS-L3-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
  • ribosome binding site of Bacillus subtilis (BBa_K5117000)
  • eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4),
    addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025) </li>
  • cotY gene of Bacillus subtilis (BBa_K5117022)
  • bidirectional terminator B0014 (BBa_B0014)

    • </u>


    Biosafety level: S1

    Target organism: Bacillus subtilis

    Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)

    Application: Degradation of cellulose


    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1669
      Illegal AgeI site found at 726
    • 1000
      COMPATIBLE WITH RFC[1000]