Difference between revisions of "Part:BBa K5401000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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No design considerations.
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The design of the plasmid contains a high-copy number plasmid backbone (BBa_J428353), T7 Promoter (BBa_K3457003), RBS (BBa_J428032), eCFP(BBa_E0020) and T7Terminator (BBa_J428091).
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+
−
 
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===Source===
 
===Source===
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Molecular cloning using ligation; sequence of individual parts were derived from iGEM Registry.
 
Molecular cloning using ligation; sequence of individual parts were derived from iGEM Registry.
  
 
===References===
 
===References===

Latest revision as of 14:50, 1 October 2024


pUC_T7Promoter_eCFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2492
    Illegal suffix found in sequence at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2492
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 2498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2492
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2492
    Illegal suffix found in sequence at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2492
    Illegal XbaI site found at 2507
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2188


Design Notes

The design of the plasmid contains a high-copy number plasmid backbone (BBa_J428353), T7 Promoter (BBa_K3457003), RBS (BBa_J428032), eCFP(BBa_E0020) and T7Terminator (BBa_J428091).

Source

Molecular cloning using ligation; sequence of individual parts were derived from iGEM Registry.

References