Difference between revisions of "Part:BBa K243034:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
[https://static.igem.org/mediawiki/parts/f/fc/Freiburg09_418.txt Commented GenBank file]
+
[https://static.igem.org/mediawiki/parts/f/fc/Freiburg09_418.txt Commented GenBank file]<br>
 
This vector is only a expression vector. It has the additional restriction sites AgeI and NgoMIV within the vector.
 
This vector is only a expression vector. It has the additional restriction sites AgeI and NgoMIV within the vector.
 
It has a strong RBS for a high expression level,an ampicilin resistance and lacI operon.
 
It has a strong RBS for a high expression level,an ampicilin resistance and lacI operon.

Latest revision as of 03:23, 22 October 2009

pJS418


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2267
    Illegal XbaI site found at 1383
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2268
    Illegal PstI site found at 2282
    Illegal NotI site found at 2275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1495
    Illegal BamHI site found at 2366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2268
    Illegal XbaI site found at 1383
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal suffix found in sequence at 2258
    Illegal XbaI site found at 1383
    Illegal NgoMIV site found at 1391
    Illegal NgoMIV site found at 2499
    Illegal AgeI site found at 3833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2115


Design Notes

Commented GenBank file
This vector is only a expression vector. It has the additional restriction sites AgeI and NgoMIV within the vector. It has a strong RBS for a high expression level,an ampicilin resistance and lacI operon.

Source

References