Difference between revisions of "Part:BBa K5302023"

Revision as of 14:36, 1 October 2024

pBBR-OmpA-V107

This work is derived from pBBRMCS-OmpA-mCherry and PUC19-ZVEGF-V114-V107-peptide, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and V107(approximately 2kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express V107. The plasmid uses lac promotor and has kanamycin resistence.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3979
Illegal XbaI site found at 3140
Illegal PstI site found at 1967
Illegal PstI site found at 3128
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3979
Illegal PstI site found at 1967
Illegal PstI site found at 3128
Illegal NotI site found at 1008
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3979
Illegal BglII site found at 1754
Illegal BamHI site found at 3146
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3979
Illegal XbaI site found at 3140
Illegal PstI site found at 1967
Illegal PstI site found at 3128
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3979
Illegal XbaI site found at 3140
Illegal PstI site found at 1967
Illegal PstI site found at 3128
Illegal NgoMIV site found at 2418
Illegal NgoMIV site found at 2701
Illegal NgoMIV site found at 4637
Illegal AgeI site found at 4477
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1330
Illegal SapI.rc site found at 2267
Illegal SapI.rc site found at 2477

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 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K5302002 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K5302023 SequenceAndFeatures</partinfo>
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 ===Functional Parameters===
-<partinfo>BBa_K5302002 parameters</partinfo>
+<partinfo>BBa_K5302023 parameters</partinfo>
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