Difference between revisions of "Part:BBa K5283022"

Revision as of 14:06, 1 October 2024

pGroESL-SPusp45-LEISSTCDA-Linker-LMWP-FGF21-histag Plasmid Detailed Description1. GroESL Inducible Promoter Function: The GroESL promoter is regulated by heat shock proteins and is typically activated under stress conditions, such as heat shock or chemical induction. Source: Derived from Escherichia coli heat shock protein genes, commonly utilized in industrial microbiology or synthetic biology. Application: Its inducible nature makes it suitable for controlled protein expression, particularly when expression needs to be initiated under specific conditions, aiding in maximizing protein yield and stability. 2. SPusp45 Signal Peptide Function: SPusp45 is a signal peptide sequence that directs the secretion of proteins out of the cell. Source: Typically sourced from the secretion system of Lactococcus lactis. Applicatio*: Used for secretory expression of recombinant proteins, enhancing solubility and purity, facilitating subsequent purification and application. 3. LEISSTCDA Sequence Function: LEISSTCDA is a peptide sequence used for protein modification or tagging. Source: This sequence may be obtained through in vitro selection or design, with specific functions depending on experimental requirements. Application: Employed to enhance protein functionality or facilitate detection and purification. 4. Linker (Peptide Linker) Function: Peptide linkers provide flexibility between protein domains, preventing structural interference. Source: Common linker sequences include (Gly_4Ser)_n, typically obtained through design. Application: Used to connect different functional domains, ensuring each domain can correctly fold and function independently. 5. LMWP (Low Molecular Weight Poly-Lysine) Function: LMWP is a cell-penetrating peptide sequence that aids in translocating proteins across cell membranes. Source: Generally, it is artificially designed or selected through screening. Application: Used in drug delivery systems to enhance intracellular delivery efficiency of genes or drugs. 6. FGF21 (Fibroblast Growth Factor 21) Function: FGF21 is a metabolic regulatory protein with roles in regulating glucose and lipid metabolism.**Source**: Naturally occurring in the human body and produced via gene cloning and expression systems. Application: Important in the research and treatment of metabolic diseases such as diabetes and obesity, potentially serving as a therapeutic protein drug. 7. His Tag Function: The His tag is a sequence of six histidines used for protein purification. Source: Widely utilized in molecular biology tools, added to recombinant proteins via genetic engineering. Application: Facilitates purification using nickel affinity chromatography (Ni-NTA), enabling high-purity target protein recovery. Potential Applications1. Metabolic Research: By inducing FGF21 expression, researchers can investigate its mechanisms in metabolic diseases.2. Protein Drug Development: Production and purification of FGF21 for preclinical research and drug development for metabolic disorders.3. Gene Therapy: Utilizing the cell-penetrating properties of LMWP to explore the feasibility of intracellular delivery of FGF21 genes or proteins.4. Industrial Production: Using the GroESL inducible promoter to mass-produce FGF21 in industrial microorganisms, reducing production costs Summary This plasmid combines multiple functional sequences to efficiently express and secrete FGF21 protein under specific conditions, facilitating subsequent purification and application. It holds significant potential for research and preclinical development in various fields.

Sequence and Features

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