Difference between revisions of "Part:BBa K197001:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K197001 short</partinfo>
 
<partinfo>BBa_K197001 short</partinfo>
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===Source===
 
===Source===
 +
<pre>
 +
Pool O09ig1 through O09ig26, assemble by PCA
 +
PCR O09ig1/O09ig26 on PCA reaction        (860, EcoRI/BamHI
 +
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
 +
Product is pBca9523-B09ig1  {<azo1653 AtD>}
 +
----
 +
O09ig1    PCA assembly of {<azo1653 AtD>} (B09ig1)
 +
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
 +
O09ig2    PCA assembly of {<azo1653 AtD>} (B09ig1)
 +
TAGCACCAGCGTTACCGTCGTCGTTCATACGCTGAGTGCCAGAAGACATCTGG
 +
O09ig3    PCA assembly of {?azo1653 AtD>} (B09ig1)
 +
CGACGGTAACGCTGGTGCTACCACCCAGGACTTCGACGGTGCTCTGATCGGTG
 +
O09ig4    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CAACACGCCAGTCACCGAAAGCCGGCAGGTCACCACCGATCAGAGCACCGTCG
 +
O09ig5    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
TTTCGGTGACTGGCGTGTTGGTGCTGCTATCGGTTACGACATCGCTCAGGTTT
 +
O09ig6    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
TGGTTTCACCCTCTGCCTGACGAGAACCAACAGAAACCTGAGCGATGTCGTAA
 +
O09ig7    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
TCAGGCAGAGGGTGAAACCAAAACCCGTCGTTTCGCTCTGTACTCTGGTAAAT
 +
O09ig8    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CACGCAGAGAGTTACCGTCAGCCAGTTCGAAAGATTTACCAGAGTACAGAGCG
 +
O09ig9    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
TGACGGTAACTCTCTGCGTGTTTTCGGTGGTGCTGCTTACTCTCTGCACCGTC
 +
O09ig10    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CGTCGATCAGAGAAACGTCACGAGCAGATTCCAGACGGTGCAGAGAGTAAGCA
 +
O09ig11    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
TGACGTTTCTCTGATCGACGGTGTTGAACACCTGACCCGTGGTTACGACGTTG
 +
O09ig12    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
GGTAAGCCAGTTCACCGAAGAGTTGACGGGTAACAACGTCGTAACCACGGGTC
 +
O09ig13    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CTTCGGTGAACTGGCTTACCGTCTGGGTCTGGGTGAAAAATCTCACGTTGAAC
 +
O09ig14    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CACGCTGAGCAACACGCATCAGACCAACGAACGGTTCAACGTGAGATTTTTCA
 +
O09ig15    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
GATGCGTGTTGCTCAGCGTGCTGACGGTTTCCAGGAAGAAGGTGGTTCTGCTG
 +
O09ig16    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
GCAGAGCGTTACGCTGAGAGTCACCAGCCAGAGCAGCAGAACCACCTTCTTCC
 +
O09ig17    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CTCTCAGCGTAACGCTCTGCTGATCTCTACCCTGGGTGCTCGTGGTCAGCACG
 +
O09ig18    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
TCAGTTCGATGTCCAGACCACCCAGGTTAACAGCGTGCTGACCACGAGCACCC
 +
O09ig19    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
TGGTCTGGACATCGAACTGAAAGGTTCTGTTACCTGGCGTCACCTGGAAGGTG
 +
O09ig20    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CAGACAGACGCAGACCCAGTTCCGGACGAACGTCACCTTCCAGGTGACGCCAG
 +
O09ig21    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
ACTGGGTCTGCGTCTGTCTGGTGGTGCTCGTTTCACCGTTATGGGCACTGAAC
 +
O09ig22    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CGTTCAGGGTCAGCAGCATAGAGTCACGCGGCAGTTCAGTGCCCATAACGGTG
 +
O09ig23    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
TATGCTGCTGACCCTGAACGCTAACTACGACCTGATGGCTGGTGTTGTTCTGA
 +
O09ig24    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CAGAGTCAGAGGTCATAGCGTTAACACCAACGTTCAGAACAACACCAGCCATC
 +
O09ig25    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CGCTATGACCTCTGACTCTGGTAACGCTGCTGCTCTGGTTGCTAACCTGCGCT
 +
O09ig26    PCA assembly of {?azo1653 AtD?} (B09ig1)
 +
CGTTAGGATCCGTTCATTTTCCAGCGCAGGTTAGCAACCAGA
  
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It comes from the genomic DNA of Azoarcus sp. (strain BH72)
+
</pre>
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+
 
===References===
 
===References===

Revision as of 03:14, 22 October 2009

{Azo1653 AtD}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Pool O09ig1 through O09ig26, assemble by PCA
PCR O09ig1/O09ig26 on PCA reaction        (860, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig1   {<azo1653 AtD>}
----
O09ig1     PCA assembly of {<azo1653 AtD>} (B09ig1)
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig2     PCA assembly of {<azo1653 AtD>} (B09ig1)
TAGCACCAGCGTTACCGTCGTCGTTCATACGCTGAGTGCCAGAAGACATCTGG
O09ig3     PCA assembly of {?azo1653 AtD>} (B09ig1)
CGACGGTAACGCTGGTGCTACCACCCAGGACTTCGACGGTGCTCTGATCGGTG
O09ig4     PCA assembly of {?azo1653 AtD?} (B09ig1)
CAACACGCCAGTCACCGAAAGCCGGCAGGTCACCACCGATCAGAGCACCGTCG
O09ig5     PCA assembly of {?azo1653 AtD?} (B09ig1)
TTTCGGTGACTGGCGTGTTGGTGCTGCTATCGGTTACGACATCGCTCAGGTTT
O09ig6     PCA assembly of {?azo1653 AtD?} (B09ig1)
TGGTTTCACCCTCTGCCTGACGAGAACCAACAGAAACCTGAGCGATGTCGTAA
O09ig7     PCA assembly of {?azo1653 AtD?} (B09ig1)
TCAGGCAGAGGGTGAAACCAAAACCCGTCGTTTCGCTCTGTACTCTGGTAAAT
O09ig8     PCA assembly of {?azo1653 AtD?} (B09ig1)
CACGCAGAGAGTTACCGTCAGCCAGTTCGAAAGATTTACCAGAGTACAGAGCG
O09ig9     PCA assembly of {?azo1653 AtD?} (B09ig1)
TGACGGTAACTCTCTGCGTGTTTTCGGTGGTGCTGCTTACTCTCTGCACCGTC
O09ig10     PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTCGATCAGAGAAACGTCACGAGCAGATTCCAGACGGTGCAGAGAGTAAGCA
O09ig11     PCA assembly of {?azo1653 AtD?} (B09ig1)
TGACGTTTCTCTGATCGACGGTGTTGAACACCTGACCCGTGGTTACGACGTTG
O09ig12     PCA assembly of {?azo1653 AtD?} (B09ig1)
GGTAAGCCAGTTCACCGAAGAGTTGACGGGTAACAACGTCGTAACCACGGGTC
O09ig13     PCA assembly of {?azo1653 AtD?} (B09ig1)
CTTCGGTGAACTGGCTTACCGTCTGGGTCTGGGTGAAAAATCTCACGTTGAAC
O09ig14     PCA assembly of {?azo1653 AtD?} (B09ig1)
CACGCTGAGCAACACGCATCAGACCAACGAACGGTTCAACGTGAGATTTTTCA
O09ig15     PCA assembly of {?azo1653 AtD?} (B09ig1)
GATGCGTGTTGCTCAGCGTGCTGACGGTTTCCAGGAAGAAGGTGGTTCTGCTG
O09ig16     PCA assembly of {?azo1653 AtD?} (B09ig1)
GCAGAGCGTTACGCTGAGAGTCACCAGCCAGAGCAGCAGAACCACCTTCTTCC
O09ig17     PCA assembly of {?azo1653 AtD?} (B09ig1)
CTCTCAGCGTAACGCTCTGCTGATCTCTACCCTGGGTGCTCGTGGTCAGCACG
O09ig18     PCA assembly of {?azo1653 AtD?} (B09ig1)
TCAGTTCGATGTCCAGACCACCCAGGTTAACAGCGTGCTGACCACGAGCACCC
O09ig19     PCA assembly of {?azo1653 AtD?} (B09ig1)
TGGTCTGGACATCGAACTGAAAGGTTCTGTTACCTGGCGTCACCTGGAAGGTG
O09ig20     PCA assembly of {?azo1653 AtD?} (B09ig1)
CAGACAGACGCAGACCCAGTTCCGGACGAACGTCACCTTCCAGGTGACGCCAG
O09ig21     PCA assembly of {?azo1653 AtD?} (B09ig1)
ACTGGGTCTGCGTCTGTCTGGTGGTGCTCGTTTCACCGTTATGGGCACTGAAC
O09ig22     PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTTCAGGGTCAGCAGCATAGAGTCACGCGGCAGTTCAGTGCCCATAACGGTG
O09ig23     PCA assembly of {?azo1653 AtD?} (B09ig1)
TATGCTGCTGACCCTGAACGCTAACTACGACCTGATGGCTGGTGTTGTTCTGA
O09ig24     PCA assembly of {?azo1653 AtD?} (B09ig1)
CAGAGTCAGAGGTCATAGCGTTAACACCAACGTTCAGAACAACACCAGCCATC
O09ig25     PCA assembly of {?azo1653 AtD?} (B09ig1)
CGCTATGACCTCTGACTCTGGTAACGCTGCTGCTCTGGTTGCTAACCTGCGCT
O09ig26     PCA assembly of {?azo1653 AtD?} (B09ig1)
CGTTAGGATCCGTTCATTTTCCAGCGCAGGTTAGCAACCAGA

References