Difference between revisions of "Part:BBa K5291025"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | In carbon fixation module we constructed pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i> to improve the intracellular NADPH and NADP+ level.<br> | + | In carbon fixation module we constructed pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i> to improve the intracellular NADPH and NADP<sup>+</sup> level.<br> |
<html><img width = "700" src="https://static.igem.wiki/teams/5291/images/parts-lhs/pbbr1mcs2-pnta-pntb-nadm-nadk.png" /></html><br> | <html><img width = "700" src="https://static.igem.wiki/teams/5291/images/parts-lhs/pbbr1mcs2-pnta-pntb-nadm-nadk.png" /></html><br> | ||
<b>Fig.1 The map of plasmid pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i>.</b><br><br> | <b>Fig.1 The map of plasmid pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i>.</b><br><br> | ||
Revision as of 13:38, 1 October 2024
pntA: α subunit of membrane-bound proton-translocating pyridine nucleotide transhydrogen
pntA encodes PntA, the α subunit of membrane-bound proton-translocating pyridine nucleotide transhydrogenase. It is composed of two domains, i.e. hydrophilic domain I containing the NAD(H) binding site and hydrophobic domain II containing the membrane spanning α-helices. PntA can bind to membranes and plays a key role in stabilizing the overall conformation of the cytoplasmic moiety of pyridine nucleotide transferase.
Usage and Biology
In carbon fixation module we constructed pBBR1MCS2-nadM-nadK-pntA-pntB to improve the intracellular NADPH and NADP+ level.
Fig.1 The map of plasmid pBBR1MCS2-nadM-nadK-pntA-pntB.
We successfully cloned the pntA-pntB gene by PCR.
Fig.2 Electropherogram of nadM-nadK PCR gel.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]