Difference between revisions of "Part:BBa J36851:Experience"

Latest revision as of 03:08, 22 October 2009

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Applications of BBa_J36851

User Reviews

The [http://2009.igem.org/Team:Washington 2009 University of Washington iGEM Team] tried to use this part to display strepavidin on the surface of the cell, and could not determine significant binding of cells expressing this part to biotin by either flow cytometry or microscopy. For details, see this part's main page or the 2009 UW iGEM wiki.

UNIQb6f8b7352745b248-partinfo-00000000-QINU UNIQb6f8b7352745b248-partinfo-00000001-QINU

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 ===User Reviews===
+The [http://2009.igem.org/Team:Washington 2009 University of Washington iGEM Team] tried to use this part to display strepavidin on the surface of the cell, and could not determine significant binding of cells expressing this part to biotin by either flow cytometry or microscopy.  For details, see this part's main page or the 2009 UW iGEM wiki.
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+|After extensive testing of this part, we were not able to verify that binding was occurring between the streptavadin on the surface and a biotinylated flourophore we used for testing. We were able to see the protein on a protein gel, but we were not able to prove that it was correctly functioning. For more on our results, please see the [http://2009.igem.org/Team:Washington Washington iGEM 2009 page].
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