Difference between revisions of "Part:BBa K243005"
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<partinfo>BBa_K243005 short</partinfo> | <partinfo>BBa_K243005 short</partinfo> | ||
| − | This part is a linker, it can be used to connect two parts and add additional space between | + | This part is a linker, it can be used to connect two parts and add additional space between them. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our [https://parts.igem.org/Part:BBa_K157004 FluA] and [https://parts.igem.org/Part:BBa_K243003 DigA] domains, for our project the universal endonuclease. The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly . |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | Peptide linkers are used to connect protein domains. For this connection it is important that the linker itself has no influence on the connected parts. Therefore the sequence of the linker is designed for amino acids which do not interact with other amino acid residues. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.<br> | |
| + | The length of the linker is important to guarantee the independent function of two connected parts. When the linker is too short there might be a sterical interference between the parts and when it is too long, it can cause an instability of the construct. Also, it is important that the linker has a certain flexibility. <br> | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | |
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| + | <span class='h3bb'>'''Sequence and Features'''</span> | ||
<partinfo>BBa_K243005 SequenceAndFeatures</partinfo> | <partinfo>BBa_K243005 SequenceAndFeatures</partinfo> | ||
Latest revision as of 03:06, 22 October 2009
Middle Linker ( Gly-Gly-Ser-Gly)x2
This part is a linker, it can be used to connect two parts and add additional space between them. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our FluA and DigA domains, for our project the universal endonuclease. The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly .
Usage and Biology
Peptide linkers are used to connect protein domains. For this connection it is important that the linker itself has no influence on the connected parts. Therefore the sequence of the linker is designed for amino acids which do not interact with other amino acid residues. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.
The length of the linker is important to guarantee the independent function of two connected parts. When the linker is too short there might be a sterical interference between the parts and when it is too long, it can cause an instability of the construct. Also, it is important that the linker has a certain flexibility.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]