Difference between revisions of "Part:BBa K218003:Design"

(→‎Source)
(→‎Source)
 
(One intermediate revision by the same user not shown)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2  
+
LuxOU - GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2  
  
−
The source of the B0015 was the Registry of Standard Biological Parts.
+
B0015 - Registry of Standard Biological Parts
  
 
===References===
 
===References===
 +
Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.

Latest revision as of 03:05, 22 October 2009

LuxOU from Vibrio harveyi followed by terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 854
    Illegal SapI.rc site found at 816


Design Notes

LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The internal RBS was also kept, and although there is an internal terminator as well, we have constructed the strong terminator B0015 after this coding sequence in order to provide further insulation of the circuit.

Source

LuxOU - GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2

B0015 - Registry of Standard Biological Parts

References

Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.