Difference between revisions of "Part:BBa K5322002:Design"

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===References===
 
===References===
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1. Yu LC. Microbiota dysbiosis and barrier dysfunction in inflammatory bowel disease and colorectal cancers: exploring a common ground hypothesis. J. Biomed. Sci. 2018;25:79. doi: 10.1186/s12929-018-0483-8
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2.Khanna Reena, Levesque Barrett G, Sandborn William J. IBD: Measuring what counts--endoscopic assessment in IBD[J]. Nature Reviews Gastroenterology & Hepatology. 2014, 11(1): 9-10.

Latest revision as of 13:16, 1 October 2024


Constitutive Mfp5 Expression System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 300
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure successful expression of the animal-derived mussel foot protein Mfp5 in BL21(DE3), we conducted codon optimization of the Mfp5 sequence.

Source

The plasmid pET29a-J23119-RBS-Mfp5 utilizes the pET29a vector, commonly employed for robust gene expression in Escherichia coli. The components used in this design, including the J23119 promoter and Mfp5, are derived from the iGEM Registry of Standard Biological Parts (BBa_J23119, BBa_K1583002), with the mussel foot protein Mfp5 sourced from natural marine mussels. These parts were selected for their validated functionality and compatibility in synthetic biology applications, facilitating controlled expression within bacterial cells under specific conditions.

References

1. Yu LC. Microbiota dysbiosis and barrier dysfunction in inflammatory bowel disease and colorectal cancers: exploring a common ground hypothesis. J. Biomed. Sci. 2018;25:79. doi: 10.1186/s12929-018-0483-8

2.Khanna Reena, Levesque Barrett G, Sandborn William J. IBD: Measuring what counts--endoscopic assessment in IBD[J]. Nature Reviews Gastroenterology & Hepatology. 2014, 11(1): 9-10.