Difference between revisions of "Part:BBa K212005:Design"
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===References=== | ===References=== | ||
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+ | 1. Baumgartner, James W., Changhoon Kim, Renée E. Brisette, Masoyuri Inouye, Changkyu Park, and Gerald L. Hazelbauer. "Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ." Journal of Bacteriology 176: 1157-163. Journal of Bacteriology. Web. 25 July 2009. |
Latest revision as of 03:01, 22 October 2009
TrgEnvZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 348
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The genes coding for the two domains were ligated together using a shared NdeI site.
Source
Both parts come from genomic sources.
References
1. Baumgartner, James W., Changhoon Kim, Renée E. Brisette, Masoyuri Inouye, Changkyu Park, and Gerald L. Hazelbauer. "Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ." Journal of Bacteriology 176: 1157-163. Journal of Bacteriology. Web. 25 July 2009.