Difference between revisions of "Part:BBa K5322030:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The codon optimization was performed based on the codon usage table of <i>Escherichia coli</i>. | |
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===Source=== | ===Source=== | ||
− | + | PD-L1(Mus) is derived from BBa_K5322033, and this plasmid also includes BBa_J23119, BBa_B0034, and BBa_K731721. | |
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===References=== | ===References=== |
Latest revision as of 12:52, 1 October 2024
Programmed Cell Death 1 Ligand 1 [Mus musculus] Expression System
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 356
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 314
Illegal NheI site found at 939
Illegal PstI site found at 356 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 205
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 356
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 356
Illegal NgoMIV site found at 745
Illegal AgeI site found at 577 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The codon optimization was performed based on the codon usage table of Escherichia coli.
Source
PD-L1(Mus) is derived from BBa_K5322033, and this plasmid also includes BBa_J23119, BBa_B0034, and BBa_K731721.