Difference between revisions of "Part:BBa K231001"
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<partinfo>BBa_K231001 short</partinfo> | <partinfo>BBa_K231001 short</partinfo> | ||
− | Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability. The precursors for PC are naturally present in E. coli and have been shown to be upregulated in the presence of PCS and heavy metals (Li, Dhankher, Carriera. 2004). PC is a cysteine rich molecule capable of binding heavy metals. The PCS gene is endogenous to A. thaliana | + | Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability. The precursors for PC are naturally present in E. coli and have been shown to be upregulated in the presence of PCS and heavy metals (Li, Dhankher, Carriera. 2004). PC is a cysteine rich molecule capable of binding heavy metals. The PCS gene is endogenous to A. thaliana. |
Revision as of 03:00, 22 October 2009
Phytochelatin Synthase, HA-tag
Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability. The precursors for PC are naturally present in E. coli and have been shown to be upregulated in the presence of PCS and heavy metals (Li, Dhankher, Carriera. 2004). PC is a cysteine rich molecule capable of binding heavy metals. The PCS gene is endogenous to A. thaliana.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507
Illegal NgoMIV site found at 1449 - 1000COMPATIBLE WITH RFC[1000]