Difference between revisions of "Part:BBa K5233022:Experience"
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This is the plasmid map of the secretion of TurboPETase, containing our basic part turboPETase(ompA) | This is the plasmid map of the secretion of TurboPETase, containing our basic part turboPETase(ompA) | ||
https://static.igem.wiki/teams/5233/parts/turbopetase-ompa-2.png | https://static.igem.wiki/teams/5233/parts/turbopetase-ompa-2.png | ||
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After the construction of the plasmid, we successfully express the engineering E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : | After the construction of the plasmid, we successfully express the engineering E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : | ||
https://2024.igem.wiki/hust-ueve-upsaclay/protocol | https://2024.igem.wiki/hust-ueve-upsaclay/protocol | ||
Revision as of 12:48, 1 October 2024
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K5233022
This is the plasmid map of the secretion of TurboPETase, containing our basic part turboPETase(ompA)
After the construction of the plasmid, we successfully express the engineering E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : https://2024.igem.wiki/hust-ueve-upsaclay/protocol
The results of the enzyme activity is shown as follows:
User Reviews
UNIQ58a8ecaaa80caa5c-partinfo-00000000-QINU UNIQ58a8ecaaa80caa5c-partinfo-00000001-QINU