Difference between revisions of "Part:BBa K231001"
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<partinfo>BBa_K231001 short</partinfo> | <partinfo>BBa_K231001 short</partinfo> | ||
− | + | Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | The precursors for phytochelatin are naturally present in E. coli and have been shown to be upregulated in the presence of PCS and heavy metals (Li, Dhankher, Carriera. 2004). | ||
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Revision as of 02:55, 22 October 2009
Phytochelatin Synthase, HA-tag
Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 100
Illegal PstI site found at 1363
Illegal PstI site found at 1396
Illegal PstI site found at 1507
Illegal NgoMIV site found at 1449 - 1000COMPATIBLE WITH RFC[1000]