Difference between revisions of "Part:BBa K5291040"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | Given the lack of RBS on pAB after zymotic digestion, we introduced pS, a universal promoter, into the plasmid. We synthesized pS through the method of dimerization of positive and negative primers, and linked it to the plasmid backbone pAB. Then we cut off the GFP gene on pAB-pS by restriction endonuclease, gaining the usable backbone for reserved. | |
| + | <html><img width = "400" src="https://static.igem.wiki/teams/5291/images/part-wyn/ps-age-3.png" /></html> | ||
| + | <br><b>Fig.1 The AGE figure of pS.</b><br><br> | ||
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Revision as of 11:59, 1 October 2024
pS
The promoter pS is a kind of σ70-dependent constitutive promoter. It has been confirmed that pS could drive gene expression in wide range of host such as Pseudomonas putida and Azotobacter vinelandii.
Usage and Biology
Given the lack of RBS on pAB after zymotic digestion, we introduced pS, a universal promoter, into the plasmid. We synthesized pS through the method of dimerization of positive and negative primers, and linked it to the plasmid backbone pAB. Then we cut off the GFP gene on pAB-pS by restriction endonuclease, gaining the usable backbone for reserved.
Fig.1 The AGE figure of pS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]