Difference between revisions of "Part:BBa K5034205:Design"
 
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===Design Notes===
 
===Design Notes===
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We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.
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We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in ''S. oneidensis'' and regulating cellular phosphorus metabolism and electron transfer.
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+
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===Source===
 
===Source===
  
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Polyphosphate kinase 2(PPK2) from <i>Pseudomonas paraeruginosa</i>. NCBI reference sequence: NZ_CP020560.1.
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Polyphosphate kinase 2(PPK2) from <i>Pseudomonas paraeruginosa</i>. NCBI reference sequence: NZ_CP020560.1:c164262-163366
  
 
===References===
 
===References===

Latest revision as of 11:45, 1 October 2024


PolyP <->Pi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in S. oneidensis and regulating cellular phosphorus metabolism and electron transfer.

Source

Polyphosphate kinase 2(PPK2) from Pseudomonas paraeruginosa. NCBI reference sequence: NZ_CP020560.1:c164262-163366

References

Zhang, H., Ishige, K., & Kornberg, A. (2002). A polyphosphate kinase (PPK2) widely conserved in bacteria. Proceedings of the National Academy of Sciences, 99(26), 16678-16683. doi:10.1073/pnas.262655199

Neville, N., Roberge, N., & Jia, Z. (2022). Polyphosphate Kinase 2 (PPK2) Enzymes: Structure, Function, and Roles in Bacterial Physiology and Virulence. International Journal of Molecular Sciences, 23(2), 670. doi:10.3390/ijms23020670