Difference between revisions of "Part:BBa K5226085"

 
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K5226085 SequenceAndFeatures</partinfo>
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<h2>Introduction</h2>
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In order to <b>produce multiple classes of high value-added bioproducts while absorbing assimilated CDE</b>, based on the current research of related literature, we iGEM24-SCUT-China-A chose to <b>introduce the synthesis pathway of PHA particle-associated protein.</b>
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<br>
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<h2>Usage and Biology</h2>
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The SOD gene produces an antioxidant enzyme that converts harmful superoxide radicals into less active hydrogen peroxide, protecting cells from oxidative damage. To test the ability of Halomonas TD to produce phaP proteins, we chose to use the inducible promoter Mmp1. In this experiment given, we added 50 mg/L of IPTG to the flask fermentation system for induced fermentation.
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<h2>Experimental characterisation</h2>
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<h3>growth conditions</h3>
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<h3>shake flask studies</h3>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<h3>experimental design</h3>
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<h3>Data Processing and Analysis</h3>
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The fermentation broth was centrifuged to obtain the supernatant and precipitate after cell disruption, and they were subjected to SDS-PAGE, and the following results were obtained after shooting: from left to right, the protein results obtained by fermentation under two carbon sources, sodium acetate and glucose, were phaP, TD80 control, SOD, Amalase, Meta and sodium acetate and glucose. It can be seen that compared with the control group, the TD80 after the introduction of protein synthesis pathway genes can produce phaP and SOD proteins, and the protein produced with glucose as the carbon source is more than that with sodium acetate as the carbon source.
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<html> <img src="https://static.igem.wiki/teams/5226/parts/sds-page-figure-sds-phap.jpg" width="700px">
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<h2>References</h2>
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[1] Engineering Halomonas spp. as A Low-Cost Production Host for Production of Bio-surfactant Protein PhaP
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<br>
 
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===Functional Parameters===
 
===Functional Parameters===
 
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Revision as of 11:45, 1 October 2024

Mmp1-SOD

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 665
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 665
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 665
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 665
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 665
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction


In order to produce multiple classes of high value-added bioproducts while absorbing assimilated CDE, based on the current research of related literature, we iGEM24-SCUT-China-A chose to introduce the synthesis pathway of PHA particle-associated protein.

Usage and Biology

The SOD gene produces an antioxidant enzyme that converts harmful superoxide radicals into less active hydrogen peroxide, protecting cells from oxidative damage. To test the ability of Halomonas TD to produce phaP proteins, we chose to use the inducible promoter Mmp1. In this experiment given, we added 50 mg/L of IPTG to the flask fermentation system for induced fermentation.

Experimental characterisation

growth conditions



shake flask studies

experimental design

Data Processing and Analysis

The fermentation broth was centrifuged to obtain the supernatant and precipitate after cell disruption, and they were subjected to SDS-PAGE, and the following results were obtained after shooting: from left to right, the protein results obtained by fermentation under two carbon sources, sodium acetate and glucose, were phaP, TD80 control, SOD, Amalase, Meta and sodium acetate and glucose. It can be seen that compared with the control group, the TD80 after the introduction of protein synthesis pathway genes can produce phaP and SOD proteins, and the protein produced with glucose as the carbon source is more than that with sodium acetate as the carbon source.

References

[1] Engineering Halomonas spp. as A Low-Cost Production Host for Production of Bio-surfactant Protein PhaP