Difference between revisions of "Part:BBa K5034206:Design"
 
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===Design Notes===
 
===Design Notes===
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In order to improve the efficiency of energy production and phosphorus accumulation of Shewanella, this element of E. coli was migrated to Shewanella. We believe that the introduction of NADP can promote electron transport in Shewanella.
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In order to improve the efficiency of energy production and phosphorus accumulation of ''S. oneidensis'', this element of ''E. coli'' was migrated to ''S. oneidensis''. We believe that the introduction of ''NADK'' can promote electron transport in ''S. oneidensis''.
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This part is obtained by using a chemical synthesis method, with the aim of expressing the protein in ''S. oneidensis'' and regulating cellular phosphorus metabolism and electron transfer.
  
 
===Source===
 
===Source===
  
−
Inorganic polyphosphate/ATP-NAD kinase(PPNK) from <i>Mycobacterium tuberculosis H37Rv</i>. NCBI reference sequence: NC_000962.3.
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Inorganic polyphosphate/ATP-NAD kinase(PPNK) from <i>Mycobacterium tuberculosis H37Rv</i>. NCBI reference sequence: NC_000962.3:1918746-1919669
  
 
===References===
 
===References===
  
 
Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004
 
Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004

Latest revision as of 11:45, 1 October 2024


Poly P -> NADP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to improve the efficiency of energy production and phosphorus accumulation of S. oneidensis, this element of E. coli was migrated to S. oneidensis. We believe that the introduction of NADK can promote electron transport in S. oneidensis.

This part is obtained by using a chemical synthesis method, with the aim of expressing the protein in S. oneidensis and regulating cellular phosphorus metabolism and electron transfer.

Source

Inorganic polyphosphate/ATP-NAD kinase(PPNK) from Mycobacterium tuberculosis H37Rv. NCBI reference sequence: NC_000962.3:1918746-1919669

References

Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004