Difference between revisions of "Part:BBa K5034209:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.
+
We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in ''S. oneidensis'' and regulating cellular phosphorus metabolism and electron transfer.
  
  
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===Source===
 
===Source===
  
−
We synthesized the fragment using a chemical synthesis method.
+
Endopolyphosphatase(PPN1) from <i>Saccharomyces cerevisiae</i>. NCBI reference sequence: NC_001136.10.
  
 
===References===
 
===References===
 +
 +
Lichko, L. P., Kulakovskaya, T. V., & Kulaev, I. S. (2006). Inorganic polyphosphate and exopolyphosphatase in the nuclei of Saccharomyces cerevisiae: dependence on the growth phase and inactivation of the PPX1 and PPN1 genes. Yeast, 23(10), 735-740. doi:10.1002/yea.1391

Latest revision as of 11:45, 1 October 2024


Poly P -> Poly P(smaller) or Pi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in S. oneidensis and regulating cellular phosphorus metabolism and electron transfer.


Source

Endopolyphosphatase(PPN1) from Saccharomyces cerevisiae. NCBI reference sequence: NC_001136.10.

References

Lichko, L. P., Kulakovskaya, T. V., & Kulaev, I. S. (2006). Inorganic polyphosphate and exopolyphosphatase in the nuclei of Saccharomyces cerevisiae: dependence on the growth phase and inactivation of the PPX1 and PPN1 genes. Yeast, 23(10), 735-740. doi:10.1002/yea.1391