Difference between revisions of "Part:BBa K5034210:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.
+
We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in ''S. oneidensis'' and regulating cellular phosphorus metabolism and electron transfer.
  
  
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===Source===
 
===Source===
  
−
We synthesized the fragment using a chemical synthesis method.
+
Exopolyphosphatase(PPX1) from Saccharomyces cerevisiae. Codon optimization performed to express in prokaryote.
  
 
===References===
 
===References===
 +
 +
Lichko, L. P., Kulakovskaya, T. V., & Kulaev, I. S. (2006). Inorganic polyphosphate and exopolyphosphatase in the nuclei of Saccharomyces cerevisiae: dependence on the growth phase and inactivation of the PPX1 and PPN1 genes. Yeast, 23(10), 735-740. doi:10.1002/yea.1391

Latest revision as of 11:44, 1 October 2024


PolyP ---> Pi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in S. oneidensis and regulating cellular phosphorus metabolism and electron transfer.


Source

Exopolyphosphatase(PPX1) from Saccharomyces cerevisiae. Codon optimization performed to express in prokaryote.

References

Lichko, L. P., Kulakovskaya, T. V., & Kulaev, I. S. (2006). Inorganic polyphosphate and exopolyphosphatase in the nuclei of Saccharomyces cerevisiae: dependence on the growth phase and inactivation of the PPX1 and PPN1 genes. Yeast, 23(10), 735-740. doi:10.1002/yea.1391