Difference between revisions of "Part:BBa K5226084"

 
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
  
−
__NOTOC__
 
 
<partinfo>BBa_K5226084 short</partinfo>
 
<partinfo>BBa_K5226084 short</partinfo>
 +
<h2>Sequence and Features</h2>
 +
<partinfo>BBa_K5226084 SequenceAndFeatures</partinfo>
 +
<html>
 +
<body>
 +
<h2>Introduction</h2>
 +
<p>
 +
<br>
 +
In order to <b>produce multiple classes of high value-added bioproducts while absorbing assimilated CDE</b>, based on the current research of related literature, we iGEM24-SCUT-China-A chose to <b>introduce the synthesis pathway of PHA particle-associated protein.</b>
 +
<br>
 +
<h2>Usage and Biology</h2>
 +
The phaP gene produces a protein that <b>binds to the surface of hydrophobic polyhydroxyalkanoate (PHA) particles and plays an important role in regulating PHA synthesis and possibly protein folding.<b> To test the ability of <i>Halomonas</i> TD to produce phaP protein, we chose to use the inducible promoter Mmp1. In this experiment given, we added 50 mg/L of IPTG to the shake flask fermentation system for induced fermentations
 +
<br>
 +
<h2>Experimental characterisation</h2>
 +
<p>
 +
<html>
 +
<body>
 +
<h3>growth conditions</h3>
 +
<html> <img src="https://static.igem.wiki/teams/5226/parts/bba-k5226060-mmp1-am1-c1m-2.jpg" width="700px">
 +
</html>
 +
<br>
 +
<br>
 +
<html> <img src="https://static.igem.wiki/teams/5226/parts/bba-k5226060-mmp1-am1-c1m-3.jpg" width="700px">
 +
</html>
  
−
1
+
<h3>shake flask studies</h3>
−
 
+
<html> <img src="https://static.igem.wiki/teams/5226/parts/bba-k5226060-mmp1-am1-c1m-4.jpg" width="700px">
−
<!-- Add more about the biology of this part here
+
</html>
−
===Usage and Biology===
+
−
 
+
−
<!-- -->
+
−
<span class='h3bb'>Sequence and Features</span>
+
−
<partinfo>BBa_K5226084 SequenceAndFeatures</partinfo>
+
  
 +
<h3>experimental design</h3>
 +
<html> <img src="https://static.igem.wiki/teams/5226/parts/experiment-design-of-protein.png" width="700px">
 +
</html>
  
 +
<h3>Data Processing and Analysis</h3>
 +
The fermentation broth was centrifuged to obtain the supernatant and precipitate after cell disruption, and they were subjected to SDS-PAGE, and the following results were obtained after shooting: from left to right, the protein results obtained by fermentation under two carbon sources, sodium acetate and glucose, were phaP, TD80 control, SOD, Amalase, Meta and sodium acetate and glucose. It can be seen that compared with the control group, the TD80 after the introduction of protein synthesis pathway genes can produce phaP and SOD proteins, and the protein produced with glucose as the carbon source is more than that with sodium acetate as the carbon source.
 +
<br>
 +
<html> <img src="https://static.igem.wiki/teams/5226/parts/histogram-of-protein-data-results-sod-phap.jpg" width="700px">
 +
</html>
 +
<br>
 +
<html> <img src="https://static.igem.wiki/teams/5226/parts/sds-page-figure-sds-phap.jpg" width="700px">
 +
</html>
 +
<h2>References</h2>
 +
[1] Engineering Halomonas spp. as A Low-Cost Production Host for Production of Bio-surfactant Protein PhaP
 +
<br>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K5226084 parameters</partinfo>
 
<partinfo>BBa_K5226084 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 11:40, 1 October 2024

Mmp1-phaP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction


In order to produce multiple classes of high value-added bioproducts while absorbing assimilated CDE, based on the current research of related literature, we iGEM24-SCUT-China-A chose to introduce the synthesis pathway of PHA particle-associated protein.

Usage and Biology

The phaP gene produces a protein that binds to the surface of hydrophobic polyhydroxyalkanoate (PHA) particles and plays an important role in regulating PHA synthesis and possibly protein folding. To test the ability of Halomonas TD to produce phaP protein, we chose to use the inducible promoter Mmp1. In this experiment given, we added 50 mg/L of IPTG to the shake flask fermentation system for induced fermentations

Experimental characterisation

growth conditions



shake flask studies

experimental design

Data Processing and Analysis

The fermentation broth was centrifuged to obtain the supernatant and precipitate after cell disruption, and they were subjected to SDS-PAGE, and the following results were obtained after shooting: from left to right, the protein results obtained by fermentation under two carbon sources, sodium acetate and glucose, were phaP, TD80 control, SOD, Amalase, Meta and sodium acetate and glucose. It can be seen that compared with the control group, the TD80 after the introduction of protein synthesis pathway genes can produce phaP and SOD proteins, and the protein produced with glucose as the carbon source is more than that with sodium acetate as the carbon source.

References

[1] Engineering Halomonas spp. as A Low-Cost Production Host for Production of Bio-surfactant Protein PhaP