Difference between revisions of "Part:BBa K218000:Design"

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===Design Notes===
 
===Design Notes===
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This part was submitted to the Registry in 2008 (K131015); however, this part was cloned again and sequenced and is being re-submitted.  
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This part was submitted to the Registry in 2008 ([[Part:BBa_K131015]]); however, this part was cloned again and sequenced and is being re-submitted.  
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LuxP and LuxQ are found in the operon, thus we decided to keep them together. We kept natural RBS in front of LuxP, as well as the intrinsic terminator. Silent mutations were performed on a EcoRI site located within LuxP, EcorI site within LuxQ, and XbaI site within LuxQ.
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LuxP and LuxQ are found in the operon, thus we decided to keep them together. We kept natural RBS in front of LuxP, as well as the intrinsic terminator. Silent mutations were performed on a EcoRI site located within LuxP, EcorI site within LuxQ, and XbaI site within LuxQ.
  
 
===Source===
 
===Source===

Latest revision as of 02:51, 22 October 2009

LuxPQ from Vibrio harveyi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2209
    Illegal BglII site found at 2427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3509
    Illegal SapI.rc site found at 2177


Design Notes

This part was submitted to the Registry in 2008 (Part:BBa_K131015); however, this part was cloned again and sequenced and is being re-submitted.

LuxP and LuxQ are found in the operon, thus we decided to keep them together. We kept natural RBS in front of LuxP, as well as the intrinsic terminator. Silent mutations were performed on a EcoRI site located within LuxP, EcorI site within LuxQ, and XbaI site within LuxQ.

Source

GenBank Accession: NC_009784 Vibrio harveyi ATCC BAA-1116 chromosome II, REGION: 579654..580751.

References

Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM. Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing. Cell. 2006 Sep 22;126(6):1095-108.