Difference between revisions of "Part:BBa K5302021"

 
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This work is derived from pBBR1MCS-OmpA-mCherry and pUC19-miniZ-Z3C, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and miniZ(3.8kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express miniZ. The plasmid uses lac promotor and has kanamycin resistence.
  
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-pominiz-1.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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            <b>Figure 1. </b> Colony PCR results of pBBR-OmpA-miniZ       
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-pominiz-2.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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        <caption>
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            <b>Figure 2. </b> SDS-PAGE analysis of pBBR1MCS-OmpA-miniZ expression in Escherichia coli Nissle 1917 （1）     
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    <img src="https://static.igem.wiki/teams/5302/images/part-registry-pominiz-3.png"
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        width="60%" style="display:block; margin:auto;" alt="Jamboree Program" >
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    <div style="text-align:center;">
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        <caption>
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            <b>Figure 3. </b> SDS-PAGE analysis of pBBR1MCS-OmpA-miniZ expression in Escherichia coli Nissle 1917 （2）       
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Revision as of 11:02, 1 October 2024

This work is derived from pBBR1MCS-OmpA-mCherry and pUC19-miniZ-Z3C, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and miniZ(3.8kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express miniZ. The plasmid uses lac promotor and has kanamycin resistence.

Jamboree Program
Figure 1. Colony PCR results of pBBR-OmpA-miniZ

Jamboree Program
Figure 2. SDS-PAGE analysis of pBBR1MCS-OmpA-miniZ expression in Escherichia coli Nissle 1917 （1）

Jamboree Program
Figure 3. SDS-PAGE analysis of pBBR1MCS-OmpA-miniZ expression in Escherichia coli Nissle 1917 （2）