Difference between revisions of "Part:BBa K243017"

 
 
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<partinfo>BBa_K243017 short</partinfo>
 
<partinfo>BBa_K243017 short</partinfo>
  
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These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to a anticalin linked oligo.
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This part is the counter part to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243010 BBa_K243010], it inhabits the active cutting site of our universal endonuclease.
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We prefer this part because it has another combiantion of basic parts than His-FluA-Split-Fok_i, which allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication]
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The purification is made with a Streptavidin column and the linkage is done by digoxigenin binding.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This composite part is one part of our universal endonuclease and it needs another composite part like [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243010 BBa_K243010] to built to a functional heterodimer. The DigA tag leads the part to DNA which is hybridized with an digoxigenin labeled oligonucleotide. The split linker makes a distance from 51bp between the DigA and the linked Fok_a protein domain. The Strep-Tag serve as purification tag for Streptavidin column purification.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K243017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K243017 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:44, 22 October 2009

Strep-DigA-Split Linker-Fok_a

This part is the counter part to BBa_K243010, it inhabits the active cutting site of our universal endonuclease. We prefer this part because it has another combiantion of basic parts than His-FluA-Split-Fok_i, which allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication] The purification is made with a Streptavidin column and the linkage is done by digoxigenin binding.

Usage and Biology

This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243010 to built to a functional heterodimer. The DigA tag leads the part to DNA which is hybridized with an digoxigenin labeled oligonucleotide. The split linker makes a distance from 51bp between the DigA and the linked Fok_a protein domain. The Strep-Tag serve as purification tag for Streptavidin column purification.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1132